ELISA
Download our ELISA brochure (PDF)
Enzyme Linked Immunosorbent Assay (ELISA) screening techniques are widely utilized by toxicologists to screen forensic specimens for drugs of abuse. These immunoassays are extremely flexible and have adequate sensitivity to go down to the drug levels found in most forensic matrices.
• Antibody is immobilized on micro-plate well
• Competition between drug in forensic sample and enzyme labeled drug for antibody
binding sites
• The unbounded material is washed out
• Chromogenic substrate added to develop color with bound enzyme
• Resulting color is read in a spectrophotometer
PRINCIPLE OF ELISA
ADVANTAGES OF ELISA
• Heterogeneous assays maximize sensitivity
• Oriented antibody
• Optimal enzyme conjugates
• Low sample volume
• Long shelf life (minimum one year)
• “Ready to use” reagents
• No azide or mercury preservatives
• Different levels of automation